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Polyclonal Antibody

Compared with monoclonal antibody production, polyclonal antibody has the advantages of short production period and low cost. HongTide can provide polyclonal antibody preparation service with mang species including mice, mouse, rabbit, goat etc. HongTide has extensive experience in antigen design and preparation, which make the antibodies we manufactured has higher purity, and the conformation is more closely as the natural antibody. We promise the ELISA of protein antigen antibody > 1:128000, and the ELISA of peptide antigen antibody > 1:64000.

Service Content
Customer provide: Protein/peptide antigen(or use HongTide Custom Peptide Synthesis Service Deliverables:
  • >3mg Antibodies;

  • Progarmme report.

Timeline: 2 monthes.
Service Flow
Procedure
Antigen Preparation
Cell Fusion and Screening
Collecting Serum
Antibody Purification
ELISA test and antibody delivery
Description
  • Primary immunization

  • Booster immunization three times after primay immunization

  • Collect serum for each immunization

  • ELISA test for serum from the primay immunization

  • Antigen affinity purification

  • Protein A purification

  • ELISA test >1:64000(peptide antigen), >128000(protein antigen)

  • Antibody derlivery 3-8mg

Timeline
Around 2 monthes
Service Advantages
  • Guaranteed results: Positive western blot result and high ELISA titer (ELISA titer is up to 1:64,000 and 1:128,000 using peptide antigen and soluble protein antigen respectively);

  • Complete support: We pride ourselves in providing support of all steps in polyclonal antibody production: from peptide design to animal immunization, serum collection, and final antibody purification;

  • Customizable: Our project developer will work with you to develop the polyclonal antibody project. The protocol can be customized for each project to meet the specific needs of each customer.