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Isotope Labeling

Stable isotope-labeled peptides (SIL peptides) is developed for the use in Quantitative Mass Spectrometry and NMR analyses. These are excellent internal standards when quantifying analytes at very low concentrations in complex sample mixtures. Stable isotope-labeled peptides (SIL peptides) are chemically and physically indistinguishable from their endogenous counterparts with respect to retention time, ionization efficiency, and fragmentation pathways. Therefore, either are ideal internal standards and template analytes. HongTide offer customer support regarding the selection of the heavy-labeled amino acids to fit the application and the delta mass expected. Here are stable isotope-labeled amino acids we usually use during the peptide synthesis below:

Amino acids Name

Alanine / Ala(A)

Arginine / Arg(R)

Arginine / Arg(R)

Isoleucine / Ile(I)

Leucine / Leu(L)

Lysine / Lys(K)

Proline / Pro(P)

Phenyalanine / Phe(F)

Valine / Val(V)

Isotope

U-13C3,U-15N

U-13C6,U-15N4

4,4,5,5-13C

U-13C6,U-15N

U-13C6,U-15N

U-13C6,U-15N2

U-13C5,U-15N

U-13C9,U-15N

U-13C5,U-15N

Mass Difference

+4 da

+10 da

+4 da

+7 da

+7 da

+8 da

+6 da

+10 da

+6 da

sotopic enrichment

>99%

>99%

>98%

>99%

>99%

>99%

>99%

>99%

>99%

Feature

  • Tailored mass increase.

  • Strict QC test for Mass and HPLC purity.

  • 13C、15N isotope-labeled peptides.

  • Purity>95%

SIL Peptide Applications

  • Functional quantitative proteomics.

  • Quantitation of post translationally-modified proteins.

  • Protein structure analysis.

  • Protein expression monitoring.

  • Biomarker discovery.

  • Pharmacokinetics.

  • Metabolomics.

  • Clinical biochemistry for drug and metabolite monitoring.

  • Anti-doping testing.

  • Cell signal profiling and pathway validation.

  • Protein cross-linking analysis.

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