多肽FRET标记技术
FRET过程示意图
Caspase-1水解底物多肽产生荧光的过程
供体(荧光染料)
Trp (Tryptophan)
Trp (Tryptophan)
Mca (7-Methoxycoumarin-4-yl)acetyl)
Abz (2-Aminobenzoyl)
Abz (2-Aminobenzoyl)
Abz (2-Aminobenzoyl)
EDANS (5-[(2-Aminoethyl) amino]naphthalene-1-sulfonic acid)
Lucifer Yellow
FITC (Fluorescein isothiocyanate)
Dansyl (5-(Dimethylamino) naphthalene-1-sulfonyl)
5-TAMRA (Carboxytetramethyl rhodamine)
Eu(III) Chelate
颜色
最大吸收波长
280nm
280nm
325nm
320nm
320nm
320nm
340nm
430nm
490nm
342nm
547nm
340nm
最大发射波长
360nm
360nm
392nm
420nm
420nm
420nm
490nm
520nm
520nm
562nm
573nm
613nm
受体(淬灭基团)
Dnp (2,4-Dinitrophenyl)
4-Nitro-Z (4-Nitro-benzyloxycarbonyl)
Dnp (2,4-Dinitrophenyl)
pNA (para-Nitroaniline)
3-Nitro-Tyr (3-Nitro-tyrosine)
4-Nitro-Phe (4-Nitro-phenylalanine)
Dabcyl (4-(4-Dimethylaminophenylazo)benzoyl)
Dabsyl (4-(4-Diethylaminophenylazo)-benzenesulfonyl)
Dnp (2,4-Dinitrophenyl)
4-Nitro-Phe (4-Nitro-phenylalanine)
QSY7
QSY-7
